Genomic Characterization and Copy Number Variation of Bacillus anthracis Plasmids pXO1 and pXO2 in a Historical Collection of 412 Strains
نویسندگان
چکیده
منابع مشابه
Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR.
We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR.
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ژورنال
عنوان ژورنال: mSystems
سال: 2018
ISSN: 2379-5077
DOI: 10.1128/msystems.00065-18